This book provides detailed protocols for research in plant photomorphogenesis. The collection includes a broad range of topics including assays for shade avoidance responses, assays for light-dependent protein-protein interactions, photobody detection with immunofluorescence and the super-resolution imaging method, protein complex isolation from plants, detection of homodimer and monomer of photoreceptor UVR8 with immunoblotting analysis, assays for seedling greening, procedures for studying skotomorphogenesis, phenotypic study of photomorphogenesis at the seedling stage, expression of Cryptochrome in insect cells, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
ISBN: | 9781071613696 |
Publication date: | 4th March 2021 |
Author: | Ruohe Yin, Ling Li, Kaijing Zuo |
Publisher: | Humana Press an imprint of Springer US |
Format: | Hardback |
Pagination: | 176 pages |
Series: | Methods in Molecular Biology |
Genres: |
Botany and plant sciences Plant biology Developmental biology |