This detailed book explores the numerous applications of the yeast two-hybrid (Y2H) system, one of the most commonly used methods to detect protein-protein interactions. Beginning with an overview, the volume then continues by elucidating different methodologies to detect protein-protein interactions in yeast nucleus, membrane, cytoplasm, and bacteria, computational analyses of protein interaction networks, technical variations in yeast one-hybrid (Y1H) systems together with classical and more elaborated yeast methods to detect protein-DNA interactions, as well as protocols to analyze ternary protein interactions and RNA-protein and ligand-protein interactions. The book concludes with a section on methods in plant and mammalian cells that can be used to identify novel protein-protein and protein-DNA interactions and test (qualitatively and/or quantitatively) those observed in yeast. Written for the highly successful Methods in Molecular Biology series, chapters include thekind of detail and key implementation advice that leads to excellent results in the lab.
| ISBN: | 9781493993109 |
| Publication date: | 25th July 2019 |
| Author: | Luis OñateSánchez |
| Publisher: | Humana Press an imprint of Springer New York |
| Format: | Paperback |
| Pagination: | 355 pages |
| Series: | Methods in Molecular Biology |
| Genres: |
Biochemistry |
This detailed book explores the numerous applications of the yeast two-hybrid (Y2H) system, one of the most commonly used methods to detect protein-protein interactions. Beginning with an overview, the volume then continues by elucidating different methodologies to detect protein-protein interactions in yeast nucleus, membrane, cytoplasm, and bacteria, computational analyses of protein interaction networks, technical variations in yeast one-hybrid (Y1H) systems together with classical and more elaborated yeast methods to detect protein-DNA interactions, as well as protocols to analyze ternary protein interactions and RNA-protein and ligand-protein interactions. The book concludes with a section on methods in plant and mammalian cells that can be used to identify novel protein-protein and protein-DNA interactions and test (qualitatively and/or quantitatively) those observed in yeast.
Two-Hybrid Systems features in the following genres: Biochemistry
Paperback, Hardback. Not Available.
Two-Hybrid Systems was written by Luis OñateSánchez and published by Humana Press an imprint of Springer New York
Two-Hybrid Systems has 355 pages
Yes it is part of Methods in Molecular Biology series