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Development of Molecular Methods for Detection of Infectious Viruses in Treated Wastewater by T. Cromeans
  

Development of Molecular Methods for Detection of Infectious Viruses in Treated Wastewater

Part of the WERF Research Report Series Series

Synopsis

Development of Molecular Methods for Detection of Infectious Viruses in Treated Wastewater by T. Cromeans

Enteroviruses, hepatitis A virus (HAV) and other enteric viruses can survive wastewater treatment processes, even after chlorination, and are found in the final effluents. These viruses can be detected by cell culture techniques with observations for cytopathic effect (CPE). Recently molecular detection of viral nucleic acids has been used. Most viruses found in wastewater are RNA viruses and RT-PCR is a rapid and sensitive method to detect these single-strand RNA enteric viruses. This methodology does not distinguish between infectious and non-infectious viruses. Viruses inactivated in the treatment process can be detected but do not pose a public health threat. Methods are needed to quickly distinguish the infectious viruses from inactivated viruses, both of which may be present in effluents. In this project we investigated the use of a method that combines cell culture and molecular detection. If a sample contains viruses that replicate in cell culture even without CPE, the proof of replication can be demonstrated by the detection of a replicative form (RF) in cell culture that is only present during replication of infectious RNA viruses.

About the Author

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Book Info

Publication date

1st August 2004

Author

T. Cromeans

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Publisher

IWA Publishing

Format

Paperback
80 pages

Categories

Water supply & treatment
Technology: general issues

ISBN

9781843397229

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