This publication details the isolation of proteins from biological materials, techniques for solid-liquid separation, concentration, crystallization, chromatography, scale-up, process monitoring, product formulation, and regulatory and commercial considerations in protein production. The authors discuss the release of protein from a biological host, selectivity in affinity chromatography, precipitation of proteins (both non-specific and specific), extraction for rapid protein isolation, adsorption as an initial step for the capture of proteins, scale-up and commercial production of recombinant proteins, and process monitoring in downstream processing.
| ISBN: | 9780824707262 |
| Publication date: | 5th February 2003 |
| Author: | Rajni Hatti-Kaul |
| Publisher: | CRC Press Inc an imprint of Taylor & Francis Inc |
| Format: | Hardback |
| Pagination: | 676 pages |
| Genres: |
Analytical chemistry Biotechnology Industrial chemistry and chemical engineering |