Isolation and Purification of Proteins

by Rajni Hatti-Kaul

Part of the Biotechnology and Bioprocessing Series

Isolation and Purification of Proteins Synopsis

This publication details the isolation of proteins from biological materials, techniques for solid-liquid separation, concentration, crystallization, chromatography, scale-up, process monitoring, product formulation, and regulatory and commercial considerations in protein production. The authors discuss the release of protein from a biological host, selectivity in affinity chromatography, precipitation of proteins (both non-specific and specific), extraction for rapid protein isolation, adsorption as an initial step for the capture of proteins, scale-up and commercial production of recombinant proteins, and process monitoring in downstream processing.

Book Information

ISBN: 9780824707262
Publication date: 5th February 2003
Author: Rajni Hatti-Kaul
Publisher: CRC Press Inc an imprint of Taylor & Francis Inc
Format: Hardback
Pagination: 676 pages
Categories: Analytical chemistry, Biotechnology, Pharmaceutical technology,

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