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Protein Sequencing and Identification Using Tandem Mass Spectrometry by Michael Kinter, Nicholas E. Sherman

Protein Sequencing and Identification Using Tandem Mass Spectrometry


Part of the Wiley Series on Mass Spectrometry Series


Protein Sequencing and Identification Using Tandem Mass Spectrometry by Michael Kinter, Nicholas E. Sherman

How to design, execute, and interpret experiments for protein sequencing using mass spectrometry The rapid expansion of searchable protein and DNA databases in recent years has triggered an explosive growth in the application of mass spectrometry to protein sequencing. This timely and authoritative book provides professionals and scientists in biotechnology research with complete coverage of procedures for analyzing protein sequences by mass spectrometry, including step-by-step guidelines for sample preparation, analysis, and data interpretation. Michael Kinter and Nicholas Sherman present their own high-quality, laboratory-tested protocols for the analysis of a wide variety of samples, demonstrating how to carry out specific experiments and obtain fast, reliable results with a 99% success rate. Readers will get sufficient experimental detail to apply in their own laboratories, learn about the proper selection and operation of instruments, and gain essential insight into the fundamental principles of mass spectrometry and protein sequencing. Coverage includes: Peptide fragmentation and interpretation of product ion spectraBasic polyacrylamide gel electrophoresisPreparation of protein digests for sequencing experimentsMass spectrometric analysis using capillary liquid chromatographyTechniques for protein identification by database searchesCharacterization of modified peptides using tandem mass spectrometry And much more


[the authors] present...laboratory-tested protocols for the analysis of a wide variety of samples, providing sufficient detail for duplication of their methods... (SciTech Book News Vol. 25, No. 2 June 2001) informative, well-written and well-illustrated book with a large number of useful references... (

About the Author

MICHAEL KINTER, PhD, is an associate staff member in the Department of Cell Biology, Lerner Research Institute, Cleveland Clinic Foundation. NICHOLAS E. SHERMAN, PhD, is an assistant professor of research in the W. M. Keck Biomedical Mass Spectrometry Laboratory, Department of Microbiology, University of Virginia.

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Book Info

Publication date

9th October 2000


Michael Kinter, Nicholas E. Sherman

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John Wiley & Sons Inc an imprint of John Wiley and Sons Ltd


320 pages


Spectrum analysis, spectrochemistry, mass spectrometry
Genetics (non-medical)



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